full-length recombinant molecules of tcda and tcdb Search Results


mecp2  (Bioss)
90
Bioss mecp2
Mecp2, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oct 3 4  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology oct 3 4
Oct 3 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full-length+recombinant+molecules+of+tcda+and+tcdb/Octocrylene/pm36417852-159-13-17
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virus strains pogn2 plasmid lai liangxue lab n a fuw backbone virus plasmids  (Addgene inc)
90
Addgene inc virus strains pogn2 plasmid lai liangxue lab n a fuw backbone virus plasmids
Figure 1. The prolonged expression of OCT4 in porcine early TE is independent of the sequence variation on TFAP2 binding site within CR4 region of OCT4’s upstream regulatory sequence (A) IF shows OCT4 expression in in vivo early porcine embryos. BL: blastocyst. (B) Compared with mice blastocysts (mBL), porcine blastocysts (pBL) express a high level of OCT4 in TE for a longer period even after hatching. ‘‘*’’ in the first panel indicates the zona pellucida of a D3.5 mBL. (C) Experimental scheme: (1) <t>pOGN2</t> plasmid, which is bearing an EGFP reading box driven by porcine OCT4 3.2 kb upstream of the regulatory region, was mutated into different variants to check the effect of certain regions/sites within OCT4 transcription; (2) after plasmid injection into porcine zygotes, transcriptional activity of OCT4 regulatory region is judged by the EGFP fluores- cence in D6 blastocyst cells. (D) Neither the deletion of the TFAP2 binding site (DAP2), nor the mouse TFAP2 binding site (mAP2)- type mutation, or the bovine TFAP2 binding site (bAP2)-type mutation leads to switching off of porcine OCT4 promoter. Bar: 100 mm. See also Figure S1 and Table S2.
Virus Strains Pogn2 Plasmid Lai Liangxue Lab N A Fuw Backbone Virus Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full-length+recombinant+molecules+of+tcda+and+tcdb/FUW-SOKM+(Plasmid+%2320325)/pm36417852-159-65-79
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Image Search Results


Figure 1. The prolonged expression of OCT4 in porcine early TE is independent of the sequence variation on TFAP2 binding site within CR4 region of OCT4’s upstream regulatory sequence (A) IF shows OCT4 expression in in vivo early porcine embryos. BL: blastocyst. (B) Compared with mice blastocysts (mBL), porcine blastocysts (pBL) express a high level of OCT4 in TE for a longer period even after hatching. ‘‘*’’ in the first panel indicates the zona pellucida of a D3.5 mBL. (C) Experimental scheme: (1) pOGN2 plasmid, which is bearing an EGFP reading box driven by porcine OCT4 3.2 kb upstream of the regulatory region, was mutated into different variants to check the effect of certain regions/sites within OCT4 transcription; (2) after plasmid injection into porcine zygotes, transcriptional activity of OCT4 regulatory region is judged by the EGFP fluores- cence in D6 blastocyst cells. (D) Neither the deletion of the TFAP2 binding site (DAP2), nor the mouse TFAP2 binding site (mAP2)- type mutation, or the bovine TFAP2 binding site (bAP2)-type mutation leads to switching off of porcine OCT4 promoter. Bar: 100 mm. See also Figure S1 and Table S2.

Journal: Cell reports

Article Title: OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development.

doi: 10.1016/j.celrep.2022.111644

Figure Lengend Snippet: Figure 1. The prolonged expression of OCT4 in porcine early TE is independent of the sequence variation on TFAP2 binding site within CR4 region of OCT4’s upstream regulatory sequence (A) IF shows OCT4 expression in in vivo early porcine embryos. BL: blastocyst. (B) Compared with mice blastocysts (mBL), porcine blastocysts (pBL) express a high level of OCT4 in TE for a longer period even after hatching. ‘‘*’’ in the first panel indicates the zona pellucida of a D3.5 mBL. (C) Experimental scheme: (1) pOGN2 plasmid, which is bearing an EGFP reading box driven by porcine OCT4 3.2 kb upstream of the regulatory region, was mutated into different variants to check the effect of certain regions/sites within OCT4 transcription; (2) after plasmid injection into porcine zygotes, transcriptional activity of OCT4 regulatory region is judged by the EGFP fluores- cence in D6 blastocyst cells. (D) Neither the deletion of the TFAP2 binding site (DAP2), nor the mouse TFAP2 binding site (mAP2)- type mutation, or the bovine TFAP2 binding site (bAP2)-type mutation leads to switching off of porcine OCT4 promoter. Bar: 100 mm. See also Figure S1 and Table S2.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a peptide mapping near the N-terminus of Oct-3/4 of human origin Santa Cruz Cat# sc-8628; RRID:AB_653551 a full length human CDX2 recombinant protein Biogenex Cat# MU392A-UC; RRID:AB_2923402 Synthesized peptide derived from human GATA4 Sigma Cat# SAB4501129; RRID:AB_10745264 Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Gata6 Abcam Cat# ab22600; RRID:AB_732529 Bacterial and virus strains pOGN2 plasmid Lai Liangxue Lab N/A FUW backbone virus plasmids modified from Addgene Cat#20325 Biological samples Vitrified human blastocysts the Ji Nan People’s Hospital D5 blastocysts without gender identification Deposited data Raw data of RNA-seq This paper SRA: PRJNA882399 Experimental models: Organisms/strains Pig Pig Pig Oligonucleotides Primers (see Table S2) This paper N/A Recombinant DNA mN1-O/S introduced pOGN2 mutation This paper N/A hN1 introduced pOGN2 mutation This paper N/A pN2 mutated pOGN2 This paper N/A pN1 mutated pOGN2 This paper N/A pO mutated pOGN2 This paper N/A pS mutated pOGN2 This paper N/A Lentiviral vector TND This paper N/A TCD This paper N/A FUW-Oct4Egfp This paper N/A FUW-Cdx2Dsred This paper N/A PMX-Histag-Gata4 This paper N/A FUW-EGFP This paper N/A FUW-Gata4 This paper N/A FUW-EGFP-con.miR This paper N/A FUW-EGFP-Gata2miR This paper N/A FUW-EGFP-Gata3miR This paper N/A FUW-EGFP-Gata4miR This paper N/A vector OP for Luciferase reporter This paper N/A vector OP(mN1-O/S) for Luciferase reporter This paper N/A vector D1 for Luciferase reporter This paper N/A vector D2 for Luciferase reporter This paper N/A vector D3 for Luciferase reporter This paper N/A (Continued on next page) e1 Cell Reports 41, 111644, November 22, 2022

Techniques: Expressing, Sequencing, Binding Assay, In Vivo, Plasmid Preparation, Injection, Activity Assay, Mutagenesis

Figure 2. The active expression of OCT4 in early TE is dependent on a GATA binding site within porcine OCT4 CR4 region (A) Alignment of CR4 sequences across multiple mammalian species shows interesting variation in the N1 region. N: neighbor sequence; O: OCT4 binding site; S: SOX2 binding site. (B) After modified pOGN2 plasmid injection, EGFP expression shows that replacing the porcine OCT4/SOX2 binding site and its intimate upstream neighbor sequence N1 (pN1-O/S) with the corresponding sequence of the mouse (mN1-O/S) can switch off the activity of the OCT4 promoter in porcine TE cells. (C) Mutations at N1, N2, O, or S regions further confirmed that only the dysfunctional mutation of N1 can switch off the activity of the OCT4 promoter in porcine TE, while dysfunctional mutation at N2, O, S region, or the human and monkey type N1 (hN1) mutation cannot switch off the activity of OCT4 promoter in porcine TE. (D) Alignment of N1-O/S sequences across multiple mammalian species indicates in non-rodents there is an additional GATA binding site in accordance with the position weight matrices of conventional GATA motif. (E) PCR test for the transcription of GATA family genes throughout in vitro early porcine development. neg.: no template negative control; pos.: positive control.

Journal: Cell reports

Article Title: OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development.

doi: 10.1016/j.celrep.2022.111644

Figure Lengend Snippet: Figure 2. The active expression of OCT4 in early TE is dependent on a GATA binding site within porcine OCT4 CR4 region (A) Alignment of CR4 sequences across multiple mammalian species shows interesting variation in the N1 region. N: neighbor sequence; O: OCT4 binding site; S: SOX2 binding site. (B) After modified pOGN2 plasmid injection, EGFP expression shows that replacing the porcine OCT4/SOX2 binding site and its intimate upstream neighbor sequence N1 (pN1-O/S) with the corresponding sequence of the mouse (mN1-O/S) can switch off the activity of the OCT4 promoter in porcine TE cells. (C) Mutations at N1, N2, O, or S regions further confirmed that only the dysfunctional mutation of N1 can switch off the activity of the OCT4 promoter in porcine TE, while dysfunctional mutation at N2, O, S region, or the human and monkey type N1 (hN1) mutation cannot switch off the activity of OCT4 promoter in porcine TE. (D) Alignment of N1-O/S sequences across multiple mammalian species indicates in non-rodents there is an additional GATA binding site in accordance with the position weight matrices of conventional GATA motif. (E) PCR test for the transcription of GATA family genes throughout in vitro early porcine development. neg.: no template negative control; pos.: positive control.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies a peptide mapping near the N-terminus of Oct-3/4 of human origin Santa Cruz Cat# sc-8628; RRID:AB_653551 a full length human CDX2 recombinant protein Biogenex Cat# MU392A-UC; RRID:AB_2923402 Synthesized peptide derived from human GATA4 Sigma Cat# SAB4501129; RRID:AB_10745264 Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Gata6 Abcam Cat# ab22600; RRID:AB_732529 Bacterial and virus strains pOGN2 plasmid Lai Liangxue Lab N/A FUW backbone virus plasmids modified from Addgene Cat#20325 Biological samples Vitrified human blastocysts the Ji Nan People’s Hospital D5 blastocysts without gender identification Deposited data Raw data of RNA-seq This paper SRA: PRJNA882399 Experimental models: Organisms/strains Pig Pig Pig Oligonucleotides Primers (see Table S2) This paper N/A Recombinant DNA mN1-O/S introduced pOGN2 mutation This paper N/A hN1 introduced pOGN2 mutation This paper N/A pN2 mutated pOGN2 This paper N/A pN1 mutated pOGN2 This paper N/A pO mutated pOGN2 This paper N/A pS mutated pOGN2 This paper N/A Lentiviral vector TND This paper N/A TCD This paper N/A FUW-Oct4Egfp This paper N/A FUW-Cdx2Dsred This paper N/A PMX-Histag-Gata4 This paper N/A FUW-EGFP This paper N/A FUW-Gata4 This paper N/A FUW-EGFP-con.miR This paper N/A FUW-EGFP-Gata2miR This paper N/A FUW-EGFP-Gata3miR This paper N/A FUW-EGFP-Gata4miR This paper N/A vector OP for Luciferase reporter This paper N/A vector OP(mN1-O/S) for Luciferase reporter This paper N/A vector D1 for Luciferase reporter This paper N/A vector D2 for Luciferase reporter This paper N/A vector D3 for Luciferase reporter This paper N/A (Continued on next page) e1 Cell Reports 41, 111644, November 22, 2022

Techniques: Expressing, Binding Assay, Sequencing, Plasmid Preparation, Injection, Activity Assay, Mutagenesis, In Vitro, Negative Control, Positive Control